Author(s): Anand S, Ajantha, Shashirekha K S, Anuradha B
Urinary tract infections are associated with high morbidity and long-term complications. The major causative organisms of UTI are Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis etc. Klebsiella pneumoniae is the foremost micro-organism causing nosocomial infections including UTI. In Ayurveda micro-organisms are identified under Krimi and many drugs with Krimighna action is mentioned. But knowledge of specific drug against specific bacteria re-established by modern methods of culture and sensitivity is very less. Hence, if evidence can be developed for these Ayurveda drugs action scientifically against specific causative bacteria, then these observations would pave way for administration in patient. This is achieved through tools like culture and sensitivity in vitro. Gokshura and Guduchi is indicated in mutrakrichra and said to possess krimighna property. The present study aims to compare antibacterial activity of Gokshura and Guduchi against Klebsiella pneumoniae by urine culture and sensitivity method in Pittaja mutrakrichra (Urinary tract infection). Patients of Pittaja mutrakrichra were subjected for urine culture and those with positive results for Klebsiella pneumoniae were further used. Alcoholic extract of Gokshura and Guduchi was prepared by Soxhlet method. Further sensitivity test was performed by Agar well diffusion method and zone of inhibition was measured. Alcoholic extract of Guduchi has showed better antibacterial effect against Klebsiella pneumoniae in comparison to alcoholic extract of Gokshura in same concentration as the mean value of zone of inhibition is higher for Guduchi. Phytochemical components present in alcoholic extracts aid in antibacterial action against cell membrane of micro-organism.