AN IN VITRO STUDY OF AMELIORATIVE EFFECT OF COMBINATION OF ROOT EXTRACT OF OROXYLUM INDICUM WITH COW URINE DISTILLATE ON CYPERMETHRIN INDUCED CYTOTOXICITY ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY

Abstract

Author(s): Daya Shankar Gautam *, Mamta Gokhale, Archana Patel, Meenal Raikwar

Cypermethrin is a pesticide which is used by the farmers of Jabalpur as well as throughout the country. Farmers are exposed to it while spreading it on the crops. In the current study, effect of combination of root extract of Oroxylum indicum with cow urine distillate on cypermethrin induced cytotoxicity on human peripheral blood lymphocytes using MTT assay was assessed as cow urine and Oroxylum indicum has been found life supporting agents. Different concentration of cypermethrin were used to induce toxicity. Aqueous fraction of 10% root extract of Oroxylum indicum in combination with 50% cow urine distillate showed ameliorative effect. This combination may be used for the treatment against cypermethrin induced cytotoxicity in the farmers exposed to it.