Author(s): Balamurugan K*, Kirtimaya Mishra, Suresh R
A new and simple method was developed for quantitative determination of Vildagliptin (VILD) and Metformin (MET) spiked with plasma using an Onyx C18 Monolithic column connected with an Onyx C18 guard cartridge and PDA detection at 220 nm. The mobile phase methanol (MeOH)/acetonitrile (ACN)/potassium dihydrogen orthophosphate (KH2PO4) was pumped at 0.4 mL/min. The method was linear between 0.5-2.5 µg/mL, statistically validated for its linearity, precision, and accuracy. The precision was found to be less than 1% of the assay method. It was found that the additives in the commercial tablet did not interfere with the method. The currently developed method can routinely use for the estimation of VILD and MET related compounds from the tablet dosage form spiked with plasma.