Author(s): Sharma Amit Kumar, Dharamsi Abhay
A chromatographic system prominence consisting of quaternary solvent delivery pump, a degasser, an injector, column oven and photodiode array detector, LC 20AT series. C18 (4.6*250) mm, 5 micron column was used. The instrumental settings were a flow of 1ml/min. the injection volume was 20ul. The detection wavelength was 190 nm for all the three analytes.