ISSN (Online) : 2277-4572
Author(s): Iman Daham H. Al-Mola1and Zohair I.F.Rahemo2
Extracts of salivary glands of the two types of ticks namely, Hyalomma anatolicum anatolicum and a Rhipocephalus sanguineus sanguineus , were subjected to the test of some biochemical parameters . The total protein intensity has been estimated in salivary glands extracts in the two types, high percentage was found in H.anatolicum compared with R.sanguineus forms, (104.12±4.93) and (91.33±3.04) microgram/Cm3 respectively. The intensity of carbohydrates in salivary gland extracts was high in H.anatolicum (0.640±0.009), after comparing with a lower intensity for R.sanguineuss (0.360 ±0.004) microgram/Cm3 . The lipid intensity in salivary glands was high in R.sanguineus (245.37±8.76) compared with concentrations in H.anatolicum (244.80±9.27)microgram/Cm3 . The total isolation of protein amount in the salivary glands in both types of tick, electrophoresis technique with Poly acrylamid gel with SDS has been implied to trace the most important separated proteins. The results denotes the existence of the four protein bands in H.anatolicum, and three protein bands in R.sanguineus, and their molecular weights were recognized. The Molecular weights for the four bands were; 76.190, 53.616, 38.168, and 16.614 kDa, respectively, while the molecular weight for the three protein bands were 73.569, 51.760, and 32.779 kDa, respectively
