ISSN (Online) : 2277-4572
Author(s): G. Renuga* and K. Thamizhvanan
The research was designed to investigate Streptozotocin (STZ 55 mg/kg body weight) induced diabetic rats can be used as a model to design a drug for the treatment of diabetes. A novel compound purified from Eugenia jambolana seeds extract showed protein band on SDS PAGE which has the potential action of hypoglycemic so that it can be considered as drug for experimental groups. A single dose of purified protein was administered after induction of diabetes with Streptozotocin. The induction of diabetic in rats were checked by biochemical analysis revealed increase blood glucose levels in STZ induced rats than normal control, protein administrated showed decreased level of glucose and tend to bring down the level to control animals. Decreased in the content of reduced GSH in diabetic rats than control groups of rats were confirmed the compensation by the administration protein, this results has equal response with standard drug glibenclamide. Administrated of protein from Eugenia jambolana recovered the activities of antioxidants such as superoxide dismutase (SOD) Catalase (CAT) these enzymes activities restore to the level of normal activity in diabetic rats and protein could tends to compensate the damage in the liver and protect the liver from free radicals generated during diabetes. Western blot analysis showed the glucokinase expression increased at end of the experimental period in diabetic rats. Diabetic rats treated with administration of medicinal plant extract of Eugenia jambolana seed resulted in activation of β -cells and also restored almost normal architecture of liver was confirmed by histopathological examination
