Author(s): Chanam Melody Devi *, Pratap Kalita, Rajat Subhra Dutta
Introduction: Brinzolamide (BRZ), one of the carbonic anhydrase inhibitors, is effective in reducing intraocular pressure for glaucoma therapy mainly through decreasing the production of the aqueous humor. There are few literatures giving the insight on simultaneous estimation of brinzolamide with brimonidine but standard independent UV spectrophotometric method of estimation of brinzolamide has not been reported till time. Aim: This research was focused to develop a suitable method of validation of BRZ and acquire consistent, reliable and accurate data on linearity, detection limit, quantification limit, accuracy and precision using a UV spectrophotometer. Methodology: The UV spectrum of Brinzolamide was recorded by scanning 10 µg/ml concentration of brinzolamide prepared in ethanol within the wavelength region of 400-200 nm against ethanol as blank. Study of accuracy, precision and limit was carried for the validation of process. Result and Discussion: The absorbance maxima was recorded and found to be 254 nm. Initially, the linearity of the method was performed in a range of 4-12 µg/ml and the method showed good linearity with regression of y = 0.0231x – 0.016 (R2 = 0.9992), where x and y were Brinzolamide concentration and UV absorbance at 254 nm respectively. Intraday and interday precision revealed % relative standard deviation of 0.664% and 0.618% respectively. Method accuracy showed average % accuracy value of 101.12%. Limit of detection and limit of quantification were 0.334 µg/ml and 1.013 µg/ml respectively. Conclusion: The proposed method is simple and could be regarded as an economically viable technique in the routine quality control analysis of BRZ.