Author(s): Nada N. Al-Shawi*
The possible anti-proliferative effects of ciprofloxacin utilizing cell lines obtained from different sources [human rhabdomyosarcoma (RD) and transitional rat embryo fibroblasts (REF) – (passage89)] were studied. The present study was carried out from January 2011 to May 2011. Each of cell lines mentioned above was exposed to various concentrations of ciprofloxacin at concentrations (from 62.5 to 1000 mcg/ml) for 48hours in addition to control (zero) concentration. Four replicates were used for each data point and the results were expressed as mean ± SD. Ciprofloxacin caused significant growth inhibition on both cell lines only at 1000 mcg/ml concentration; but does not exert in vitro inhibitory effect on either human rhabdomyosarcoma (RD) or transformed rat embryo fibroblasts (REF) when assayed at concentrations of less than 1000 micrograms/ml.