Author(s): Kajaria Divya, Tripathi J.S, Tiwari S.K, Pandey B.L.
This study was aimed at evaluating the safety profile, acute and subchronic toxicity of Bharangyadi polyherbal Ayurvedic Compound in rodents, commonly used in the treatment of Bronchial Asthma. Acute Toxicity was evaluated in wister albino rats by administering orally graded doses of extract of Clerodendrum serratum, Hedychium spicatum, & Inula racemosa (Bharangyadi polyherbal drug) in the dose of 2000mg, 5000mg, 10g, & 20g/ kg body weight of animal and observed continuously for first 4h and hourly for next 12h, then 8 hourly for next 56hour (72 hour, acute toxicity study). The study had been strictly followed the OECD guidelines. Adult Wister albino rats were divided into 3 groups with 5 animals in each group and a control group, fed with doses of 50mg, 100mg & 150mg/ml twice in a day for 28 days. Effect of drug was evaluated on haematological parameter after every 7 days and histological study was done after sacrificing the animals on 30th day. The median acute toxicity value (LD50) of the extract was found to be infinite and drug was found to be totally non toxic as after ingestion of 20gm no animal found to be dead. Heamatological profile shows no abnormal elevation in level of S.Bilirubin, SGOT, SGPT, S.Urea, & S.Creatinine. The weight of animal rises steadily and appetite increases proportionately with the dose of drug. There was no abnormality found in histopathological assessment. However it was observed that long term use especially at a high dose may prove fatal.