Introduction: Multiple Sclerosis (MS) is a chronic disease of central nervous system which causes inflammation in the brain and the spinal cord, resulting in demyelination and damage to axons and nerve fibers. The cause of this disease is still unknown, and many neuroscientists are suspected that the disease is due to genetic, environmental and even infectious agents. The purpose of this study is to investigate the relationship between MS and Clostridium perfringens type B infection.
Method: A total of 39 stool and blood samples were collected from MS patients and 40 stool and serum samples from non-MS patients. Stool specimens were evaluated for the presence of epsilon toxin by rapid test? Strips for detection of Clostridium Perfringens Epsilon Toxin? .Then stool samples were cultured. Positive colonies were evaluated by biochemical and PCR tests . Polymerase chain reaction (PCR) was performed for colonies for the presence of alpha, beta and Epislone toxins and serum samples were examined for the presence of anti- Epsilon toxin using? Epsilon Toxin Serological Elisa Kit ? .
Results: Of the 79 stool samples tested in MS and non-MS patients, type B of Clostridium perfringens were not isolated. From the total of 39 stool ?snemicepsMS patients, 5 Clostridium perfringens were isolated which 80% of the total were belonged to type A and 20% of them were type C. Of the total of 40 stool samples of non-MS patients, only 4 samples of Clostridium perfringens were isolated which all of them were belonged to type A. Results also showed that anti-epilecin toxins shown in none of serum samples in the range of the disease.