Author(s): Santh Rani Thakur*, Pradeepthi Chilikuri, Bindu Pulugurtha, Lavanya Yaidikar
The present study aimed to evaluate the protective effect of flavonoid rich fraction of willow leaved Sea buckthorn (SBT) berries against cerebral ischemia reperfusion induced neurobehavioral deficits and excitotoxicity. Briefly, the rats were randomly divided into sham, ischemia- reperfusion (I/R) control, SBT (250 and 500 mg/kg) treated groups. The rats were received their respective treatment orally by gavage once daily for 7 days prior to middle cerebral artery occlusion (MCAO) for 2 h and reperfusion for 22 h. To evaluate the severity of neurobehavioral deficits, a battery of tests were employed such as neurological deficit score, grip strength score and adhesive tape removal test. The excitotoxicity mediators like glutamate and calcium were measured. The neuronal damage was quantified in terms of infarcted area, which was measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. SBT treated rats showed substantial improvement in neurobehavioral deficit, significantly inhibited the increased levels in glutamate and calcium, significantly prevented the associated neuronal damage. From our results, it can be concluded that supplementation of SBT significantly mitigated the ischemia- reperfusion induced neuronal damage by inhibiting excitotoxicity.