Author(s): Poonam Mangalorkar*, Sunita Shailajan and Padamnabhi Nagar
Taverniera cuneifolia (Roth) Ali is commonly called as jethimadh (Licorice). High-Performance Thin-Layer Chromatographic analysis was carried out for the regional variation of different regions of India, seasonal variation and species variation. The root powder was extracted with Methanol and further analysis was performed. Quantitation of β-sitosterol and lupeol was carried out for T. cuneifolia roots collected from different regions of India like Kutch, Rajkot, Jamnagar (Gujarat), Jodhpur (Rajasthan), Kurnool (Andhra Pradesh), India. The concentrations of β-sitosterol were found to be 1.98 % in Jamnagar, India, 2.64 % in Rajkot, India, 4.45 % in Andhra Pradesh, India and 1.90 % in Jodhpur, India. The concentrations of lupeol were found to be 2.44 % in Jamnagar, India, 2.21 % in Rajkot, India, 2.29 % in Kutch, India and 2.01 % in Jodhpur, India. Seasonal variation of β-sitosterol was found to be highest in monsoon (7.08 %), winter (3.89 %) and summer (2.64 %). Lupeol was found only in summer (1.42 %). Species variation of Taverniera roots showed only presence of β-sitosterol where Taverniera cuneifolia (10.04 %) and Taverniera abyssinica (6.89 %). Quantitation was carried out on HPTLC silica gel 60 F254 pre-coated plates with the mobile phase Toluene: Methanol: 8: 1 (v/v). A TLC scanner set at 366 nm in fluorescence / reflectance mode was used for quantitation.