Author(s): K Narendra, DSD Suman Joshi, M Satya Prasad, KVN Rathnakar Reddi, J Swathi, KM Sowjanya, A. Krishna Satya
Objective of the study was to screen four extracts (HETI, ACTI, METI and AQTI) of Trichodesma indicum leaves (Family: Boraginaceae) and its different solvent soluble concentrations for possible In vitro enzyme assay, In vivo anti-inflammatory activity in experimental rats. In vitro anti-inflammatory activity was evaluated by 5-Lipoxygenase Enzyme Assay; In vivo anti-inflammatory activity was determined by carrageenan induced rat paw oedema method in experimental rats. In vitro anti-inflammatory enzymatic assay of four extracts showed significant inhibition against lipoxygenase. METI has less IC 50 (133.55µg/ml) when compared to other three extracts. In In vivo analysis the methanol extracts of 200 & 400 mg/kg body weight showed significant inhibition of paw oedema by 55.61% and 71.43% (P < 0.01) respectively at 3rd hr compared to standard drug. The findings of studies demonstrated both In vitro and In vivo anti-inflammatory activity of the leaves of Trichodesma indicum.