Author(s): Vishnu C. P*, Gopikrishna S, Shashirekha K. S
Now a day clinical conditions pop up relating with Respiratory tract infections is at peak level; use of diagnostic tools like culture and sensitivity, to identify causative microorganism, its characteristics, culture these organism in vitro and check sensitivity against Vasa and Kantakari. Vasa (Adhatoda vasica) and Kantakari (Solanum xanthocarpum) are indicated in kasa and said to possess krimighna property. Hence, adoption of new approaches like Culture and sensitivity methods would strengthen existing Ayurvedic knowledge and help in achieving improved diagnostic and curative abilities. Therefore, present study is undertaken to study different features of the micro-organism Staphylococcus aureus, its laboratory diagnosis, its culture and assess Upashaya capability in vitro by sensitivity with south Indian grown Vasa and Kantakari. Preliminary phytochemical assay of drugs revealed that the presence of various chemical constituent which creates defence mechanism against Staphylococcus aureus. The present study aims to compare antibacterial activity of vasa and kantakari against staphylococcus aureus by sputum culture and sensitivity method in kaphajakasa (chronic bronchitis). Patients of kaphajakasa were subjected for urine culture and those with positive result for Staphylococcus aureus were further used. Alcoholic extract of Vasa and Kantakari was prepared by Soxhlet method further sensitivity test was performed by Agar well diffusion method and zone of inhibition was measured. On comparing the same concentrations of alcoholic extracts of Vasa and Kantakari, it was observed that Vasa is having better antibacterial action than Kantakari against Staphylococcus aureus in Kaphaja Kasa (Chronic Bronchitis).