Author(s): Govinda, Asha Sharma, Anju Dhiman*, Sandeep Singh, Nidhi
The toxic effects of arsenic were studied in leaves of arsenic Vigna radiata(cv) were examined. The hydroponic cultivation of Vigna radiata in medium (50µM) and high Arsenic (As) (100µM) stress and low (2µM) and high (5µM) kinetin levels with their corresponding combinations of both were supplied to the plant in Hoagland’s solution. The evaluation of overall growth and biomass accumulation in arsenic stress and kinetin treatment were done during the study. The physical parameters (fresh/dry weights root and shoot lengths) were monitored. The analysis of behaviour of nitrate assimilating enzymes in leaves of Vigna radiate in the presence of medium and high content of arsenic and kinetin treatments were executed. The investigation of modulation of nitrate and ammonium accumulation in as stressed Vigna radiata leaves by kinetin were recorded. The activity of nitrate reductase was inhibited by 100µM As while effect of 50µM As was insignificant. The grades showed significant accumulation of total nitrate in arsenic (50 µM and 100 µM) and kinetin (2 µM and 5 µM) doses as compared to control. The combination of arsenic and kinetin further augmented the total nitrate content notably as compare to arsenic treated plants (50 µM and 100µM).