Author(s): K. Siva Chaitanya* and Aziz Unnisa
The aim of present experiment was to develop validated UV spectroscopic method for simultaneous estimation of Omeprazole and Diclofenac in bulk and pharmaceutical formulation. Area under curve (Method 1), simultaneous equation (Method 2) and absorbance ratio (Method 3) were developed for the determination of Omeprazole and Diclofenac in their combined capsule formulation without prior separation. The solutions of standard and sample were prepared in methanol for all the methods. Quantitative determination of the drugs was performed at the wavelength ranges of 291-311 nm and 271-291 nm (method 1), at 301 and 281 nm (method 2) and 301 nm and 295 nm (method 3) for Omeprazole and Diclofenac respectively. The Proposed methods were evaluated for the different validation parameters like precision, reproducibility, linearity and accuracy as per ICH guidelines. Linearity was observed in the range of 5-25 μg/mL for Diclofenac and 1-5 μg/mL for Omeprazole with correlation coefficient of 0.998 and 0.999 for Omeprazole and Diclofenac respectively. These methods are simple, precise, sensitive and applicable for the simultaneous determination of these drugs in pure powder and formulation.