Author(s): Poonam Duhan, Sethi Neeraj, Pal Minakshi, Kaura Sushila, Parle Milind
Effect of different types of plant growth regulators has been studied on in vitro multiplication of Withania somnifera using nodal explants. Maximum survival percentage 100 % of explants was observed using nodal explants were treated with 0.4 % Streptocyclin and 0.4 % Bavistin for 60 minutes followed by 3 minutes treatment with 0.1 % HgCl2. MS medium supplemented with was found to be best for multiplication of nodal explants of Withania somnifera amongst seventeen media employed for multiplication. Maximum eight shoots/explants observed on MS basal medium supplemented with 1.5 mg l -1 BAP + 0.5 mg l-1 NAA after 45 days of culture. A maximum 77.8 % rooting was found on MS medium (half strength) supplemented with 0.5 mg l-1 NAA. Shoots with well developed roots were separated individually and transferred to plastic bag containing sand, soil and vermin-compost in ratio of 1:1:1 in the green house and was reported 100 % survival. The hardened plants were successfully transferred to field.