Author(s): Anju Dhiman*, Ratan Lal, Meenu Bhan, Bindu Dhiman, Ashima Hooda
The antimicrobial zest of hydro-alcoholic and methanolic seed extracts of Datura fastuosa L. was evaluated against three clinical bacterial strains (Escherichia coli, Staphylococcus aureus and Bacillus subtilis) and two clinical fungal strains (Candida allbicans and Aspergillus niger) by tube dilution method. Both the plant extracts were found to be active against the tested micro-organisms. The methanolic extract of D. fastuosa was found to inhibit bacterial strain, E.coli effectively with minimum bactericidal concentration (MBC) of 25µg/ml. The hydroalcoholic extract of D. fastuosa seeds was found to be more potent in terms of its bactericidal concentration for B. subtilis with both minimum inhibitory concentration (MIC) and MBC values as 25 µg/ml. Also, methanolic extract was found to be more efficient in inhibiting S. aureus having MIC 12.5 µg/ml. Hydro alcoholic and methanolic seed extracts of D. fastuosa were also evaluated for antioxidant potential using 1,1-diphenyl-1-picryl-hydrazyl radical scavenging assay, total antioxidant capacity, total amount of phenolic content, total flavonoid content, total flavonols and total proanthocyanidines content. The IC50 value using DPPH model for methanolic extract of D. fastuosa was found to be 28.34 μg/ml & for hydroalcoholic extract, 25.78 μg/ml. The observed values for total antioxidant capacity, total amount of phenolic content, total flavonoid content, total flavonols and total proanthocyanidines content for methanolic extract of D. fastuosa were found to be 6.83 mg/g, 9.97 mg/g, 6.34 mg/g, 5.37 mg/g and 1.42 mg/g of plant extract respectively and for hydroalcoholic extract, the values for the same parameters were 7.44 mg/g, 6.88 mg/g, 9.35 mg/g, 5.36 mg/g and 0.88 mg/g respectively.