Author(s): V.Sreeram*, M. V. Basaveswara Rao, A.V.D.Nagendrakumar, M.Sivanath, K.Subhasani
A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Lorazepam in tablet dosage form. An Inertsil ODS Ultrasphere 5 μm column having 4.6 mm x 15cm internal diameter in isocratic mode with mobile phase containing Methanol : Water (65 : 35) was used. The flow rate was 1.0ml/min. and effluents were monitored at 230 nm. The retention time for Lorazepam was 6.8 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. The Mean percent recovery of Lorazepam from tablet formulation was found to be 102.00 %. This method gives linear response from 0.0007812 – 0.01 mg/swab therefore the method can detect the above concentration of API 0.0035 mg/ swab required by the method. The proposed method was successfully applied for the quantitative determination of Lorazepam in tablet formulation.